Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5
Identifieur interne : 001531 ( Main/Exploration ); précédent : 001530; suivant : 001532Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5
Auteurs : Sheila M. Thomas [États-Unis] ; Robert A. Lamb [États-Unis] ; Reay G. Paterson [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1988.
English descriptors
- Teeft :
- Acad, Amino, Amino acid sequence, Amino acid sequences, Amino acids, Amino coterminal, Bethesda research laboratories, Cdna, Cdna clone, Cdna clones, Cell lysate, Cell lysates, Cell proteins, Choppin, Clone, Codon, Encoding, Experimental procedures, Fragment, Gene, Genome, Genomic, Immunoprecipitated, Immunoprecipitation, Influenza, Influenza virus, Internal initiation, Klenow, Klenow fragment, Lysate, Mature mrna, Measles virus, Methionine, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Mrna, Mumps, Mumps virus, Mutagenesis, Natl, Negative strand, Newcastle disease virus, Nontemplated, Nontemplated nucleotides, Nuclease, Nucleotide, Nucleotide sequence, Nucleotide sequences, Open reading frame, Open reading frames, Paramyxovirus, Paterson, Polymerase, Polypeptide, Proc, Promega biotec, Protein, Protein products, Rabbit reticulocyte lysate, Randall, Reading frame, Reading frames, Residue, Reticulocyte, Ribosomal frameshifting, Rna, Runoff transcripts, Sendal virus, Sequence analysis, Sequencing, Simian virus, Transcript, Transcriptase, Uninfected cell lysate, Unpublished data, Vaccinia virus, Virion, Virol, Virology, Virus, Virus genome, Vrna, Xbal, Xbal linkers.
Abstract
Summary: The “P≓ gene of the paramyxovirus SV5 encodes two known proteins, P (Mr ≈ 44,000) and V (Mr ≈ 24,000). The complete nucleotide sequence of the “P≓ gene has been obtained and is found to contain two open reading frames, neither of which is large enough to encode the P protein. We have shown that the P and V proteins are translated from two mRNAs that differ by the presence of two nontemplated G residues in the P mRNA. These two additional nucleotides convert the two open reading frames to one of 392 amino acids. The P and V proteins are amino coterminal and have 164 amino acids in common. The unique C terminus of V consists of a cysteine-rich region that resembles a cysteine-rich metal binding domain. An open reading frame that contains this cysteine-rich region exists in all other paramyxovirus “P≓ gene sequences examined, which suggests that it may have important biological significance.
Url:
DOI: 10.1016/S0092-8674(88)91285-8
Affiliations:
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Thomas</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, Molecular Biology, and Cell Biology Northwestern University Evanston, Illinois 60208</wicri:regionArea><wicri:noRegion>Illinois 60208</wicri:noRegion></affiliation></author><author><name sortKey="Lamb, Robert A" sort="Lamb, Robert A" uniqKey="Lamb R" first="Robert A." last="Lamb">Robert A. Lamb</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, Molecular Biology, and Cell Biology Northwestern University Evanston, Illinois 60208</wicri:regionArea><wicri:noRegion>Illinois 60208</wicri:noRegion></affiliation></author><author><name sortKey="Paterson, Reay G" sort="Paterson, Reay G" uniqKey="Paterson R" first="Reay G." last="Paterson">Reay G. Paterson</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, Molecular Biology, and Cell Biology Northwestern University Evanston, Illinois 60208</wicri:regionArea><wicri:noRegion>Illinois 60208</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1988">1988</date><biblScope unit="volume">54</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="891">891</biblScope><biblScope unit="page" to="902">902</biblScope></imprint><idno type="ISSN">0092-8674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Amino</term><term>Amino acid sequence</term><term>Amino acid sequences</term><term>Amino acids</term><term>Amino coterminal</term><term>Bethesda research laboratories</term><term>Cdna</term><term>Cdna clone</term><term>Cdna clones</term><term>Cell lysate</term><term>Cell lysates</term><term>Cell proteins</term><term>Choppin</term><term>Clone</term><term>Codon</term><term>Encoding</term><term>Experimental procedures</term><term>Fragment</term><term>Gene</term><term>Genome</term><term>Genomic</term><term>Immunoprecipitated</term><term>Immunoprecipitation</term><term>Influenza</term><term>Influenza virus</term><term>Internal initiation</term><term>Klenow</term><term>Klenow fragment</term><term>Lysate</term><term>Mature mrna</term><term>Measles virus</term><term>Methionine</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Monoclonal antibody</term><term>Mrna</term><term>Mumps</term><term>Mumps virus</term><term>Mutagenesis</term><term>Natl</term><term>Negative strand</term><term>Newcastle disease virus</term><term>Nontemplated</term><term>Nontemplated nucleotides</term><term>Nuclease</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Nucleotide sequences</term><term>Open reading frame</term><term>Open reading frames</term><term>Paramyxovirus</term><term>Paterson</term><term>Polymerase</term><term>Polypeptide</term><term>Proc</term><term>Promega biotec</term><term>Protein</term><term>Protein products</term><term>Rabbit reticulocyte lysate</term><term>Randall</term><term>Reading frame</term><term>Reading frames</term><term>Residue</term><term>Reticulocyte</term><term>Ribosomal frameshifting</term><term>Rna</term><term>Runoff transcripts</term><term>Sendal virus</term><term>Sequence analysis</term><term>Sequencing</term><term>Simian virus</term><term>Transcript</term><term>Transcriptase</term><term>Uninfected cell lysate</term><term>Unpublished data</term><term>Vaccinia virus</term><term>Virion</term><term>Virol</term><term>Virology</term><term>Virus</term><term>Virus genome</term><term>Vrna</term><term>Xbal</term><term>Xbal linkers</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: The “P≓ gene of the paramyxovirus SV5 encodes two known proteins, P (Mr ≈ 44,000) and V (Mr ≈ 24,000). The complete nucleotide sequence of the “P≓ gene has been obtained and is found to contain two open reading frames, neither of which is large enough to encode the P protein. We have shown that the P and V proteins are translated from two mRNAs that differ by the presence of two nontemplated G residues in the P mRNA. These two additional nucleotides convert the two open reading frames to one of 392 amino acids. The P and V proteins are amino coterminal and have 164 amino acids in common. The unique C terminus of V consists of a cysteine-rich region that resembles a cysteine-rich metal binding domain. An open reading frame that contains this cysteine-rich region exists in all other paramyxovirus “P≓ gene sequences examined, which suggests that it may have important biological significance.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Thomas, Sheila M" sort="Thomas, Sheila M" uniqKey="Thomas S" first="Sheila M." last="Thomas">Sheila M. Thomas</name></noRegion><name sortKey="Lamb, Robert A" sort="Lamb, Robert A" uniqKey="Lamb R" first="Robert A." last="Lamb">Robert A. Lamb</name><name sortKey="Paterson, Reay G" sort="Paterson, Reay G" uniqKey="Paterson R" first="Reay G." last="Paterson">Reay G. Paterson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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